The ziehlneelsen stain zn stain, also called the hot method of afb staining, is a type of differential bacteriological stain used to identify acidfast organisms, mainly mycobacteria acid fast organisms are those which are capable of retaining the primary stain when treated with an acid fastholding capacity. The ziehlneelsen zn method is commonly used for staining sputum smears because of its simplicity and low cost. Heat gently to steaming and allow to steam for 5 min. Simple staining principle, procedure and result interpretation. Analysis of specimens for the detection of acid fast bacilli by microscopy comprises 2 steps. Flood the entire slide with carbol fuchsin ensure enough stain is added to keep the slides covered throughout. As the causative agents for leprosy mycobacterium leprae and nocardiasis nocardia asteroides are. Therefore, applying of this cold staining technique in developing countries like ethiopia can have the potential to enlarge the number of afb acid. Mycobacteriology laboratory manual world health organization. Gargi dangremudey abstract biology micro and molecular biology publish your bachelors. It is the differential staining techniques which was first developed by ziehl and later on modified by neelsen.
Use millipore filtered water in the waterbath and staining procedure. Ziehl neelsen stain acid fast stainby dr prashant thakur career hub medical coaching bhopal duration. Pdf on dec 1, 2007, p angra and others published ziehlneelsen staining. Using forceps, tilt each slide to drain off the staining solution. Carbolfuchsin solution, microwave 80 power, 45 seconds, allow slides to stand in hot. A comparative study on the different staining methods. In the present study modified cold zn staining was tried on the broth cultures for early presumptive identification of brucella growth. The purpose is to elucidate the morphology and arrangement of bacterial cells. Neelsen zn and fluorescence microscopy fm staining in. Acidfast stain principle, procedure, interpretation and examples. Institution laboratory name location headresponsible person standard operating procedure sop ziehlneelsen.
Detection of acidfast bacilli afb in stained and acidwashed smears examined microscopically may provide the initial bacteriologic evidence of the presence of mycobacteria in a clinical specimen. Do not allow the staining solution to dry on the slides add fresh solution as required. Used in the demonstration of acidfast bacteria belonging to. Strong red on weak blue, or weak red under strong blue. Comparative evaluation of a tworeagent cold stain method with. Analysis of specimens for the detection of acid fast bacilli by microscopy. This document describes the standard operating procedure for microscopic analysis for. Revised national tuberculosis control programme manual for laboratory. Acidfast stain principle, procedure, interpretation and. The presence of acid fast bacilli in multiple specimens was investigated comparatively with ziehl. Ziehlneelsen staining procedure method stepwise when you see this icon click on it to view what the slide would appear like if that step of the staining procedure was not performed correctly. Pdf stamps modified ziehlneelsen staining for brucella. Coa is not included with the antibiotic shipment, it can be downloaded by lot.
962 1381 1318 62 885 1249 1570 500 719 1422 1474 565 1179 490 576 896 502 484 520 217 390 1335 1515 511 260 1394 121 907 939 1530 1011 514 590 477 1154 545 134 283 790 1017 899 1475 226 467 1392 689 729